Preparation and Characterization of Chitosan/κ-Carrageenan Based Polymeric Nanoparticles for Gemcitabine Delivery

Authors : Habibe YILMAZ

Pages : 636-648

Doi:10.18185/erzifbed.1134093

View : 23 | Download : 23

Publication Date : 2022-08-31

Article Type : Research Paper

Abstract :Cancer is the most fatal disease of the last century after cardiovascular disease. Gemcitabine is a nucleoside analogue used in the treatment of cancer. However, like many anticancer drugs, it has many side effects that limit treatment. A nano drug delivery system has been developed to provide effective treatment by reducing side effects. Chitosan and κ-carrageenan, which are regarded as safe by the FDA, were used in the preparation of the nano drug delivery system. In the synthesis, pre-ionic gelation followed by polyelectrolyte complexation method was used and then a second ionic gelation step was added. Chitosan: κ-carrageenan ratio and CaCl2 concentration were optimized and the optimum polymer ratio was determined as 6:1 and CaCl2 concentration was 2.5%. Its hydrodynamic size at optimum conditions was 393 nm and its size measured size in TEM was 20 nm. FTIR analyzes showed that nanoparticle synthesis was successful. Drug loading was performed by encapsulation and 58% drug loading was achieved. After drug loading, the hydrodynamic dimension was increased to 595 nm and its size measured size in TEM 45 nm. Drug release was monitored for 95 hours and was determined to be higher at pH 6.0 compared to 7.4 and pH sensitive. In addition, the Higuchi model is the most suitable mathematical model for drug release kinetics. The obtained results showed that chitosan: κcarrageenan nanoparticles were suitable for gemcitabine delivery and were pH sensitive enough to respond to the tumor microenvironment.
Keywords : chitosan, kappa carrageenan, gemcitabine, drug delivery, nanoparticle, chitosan, kappa carrageenan, gemcitabine, drug delivery, nanoparticle