Impact of Heterocyclic Benzimidazole–Thioether Derivatives on the Proliferation of Human Dermal Fibroblast

Benzimidazole derivatives have attracted considerable attention in anticancer research due to their favorable pharmacological and physicochemical properties. However, data regarding their cytotoxicity and safety in normal (non-cancerous) cell lines are still limited. Therefore, in this study, the effect of heterocyclic benzimidazole-thioether compounds on the proliferation of Human Skin Fibroblast (HDFa) cell line was investigated at concentrations of 10 nM, 100 nM, 500 nM, 1 µM and for 24 and 48 hours. IC50 values for heterocyclic benzimidazole-thioether compounds 11 and 12 were found to be 117.93, 13.2 nM and 52.14, 28.2 nM for 24 and 48 hours, respectively. In the study, the closest viability concentrations to the control group for the 11 molecule among the heterocyclic benzimidazole-thioether compounds were found to be 10nm at 24 and 48 hours. In addition, while 500 nm and 1 µM were statistically significant at 24 hours for compound-11, 100 nm, 500 nm and 1 µM were statistically significant at 24 hours for compound-12, while all concentrations were statistically significant at 48 hours (p<0.05).