Detection of a Mixed Infection of Lumpy Skin Disease Virus and Foot and Mouth Disease Virus in a Calf

Authors : Murat ŞEVİK

Pages : 69-72

Doi:10.35864/evmd.516688

View : 20 | Download : 5

Publication Date : 2017-12-15

Article Type : Other Papers

Abstract :In this study, I investigated the possibility of dual infection with LSDV and FMDV in a 6-month-old Holstein calf which had fever, limping, nasal secretions, and lesions on the skin and vesicles on mucous membranes of the mouth. To assess presence of LSDV DNA in skin lesions, swab samples from skin were collected, and were analysed by real time PCR. Epithelium samples were collected from vesicular lesions, and were tested by the Sap Institute, Ankara for FMDV infection. Genetic characterization of the LSDV field isolate was conducted by sequencing the G-protein-coupled chemokine receptor gene segment. LSDV DNA was detected in swab samples and calf was diagnosed with FMD, serotype A. Phylogenetic analysis showed that the field isolate in this study was clustered together with other Africa, Europe and Middle East isolates. To the best of my knowledge, this is the first report on the dual infection of a calf with LSDV and FMDV.
Keywords : Lumpy skin disease virus, Foot and mouth disease virus, Dual infection, calf