Glycosides Isolated from Leaf Extract of Phyllanthus muellerianus (Kuntze) Excell (Phyllanthaceae) Upregulated Cell-mediated Innate Immunity

Authors : Martha Nneoma OFOKANSİ, Ogechukwu N ISİOGUGU, Ikechukwu E PETER, Matthas AGBO, Festus Bc OKOYE, Peter A AKAH

Pages : 11-24

Doi:10.55262/fabadeczacilik.1083066

View : 57 | Download : 15

Publication Date : 2023-03-01

Article Type : Research Paper

Abstract :Intracellular pathogens are mainly eliminated by cell-mediated immunity offered by phagocytic cells like neutrophils, macrophages and monocytes. Two tannin glycosides, 1-O-galloyl-6-O-luteoyl-α-D-glucopyranoside insert ignore into journalissuearticles values(1); and 3-O-methylellagic acid 3\`-O-α-rhamnopyranoside insert ignore into journalissuearticles values(2); were isolated from the ethyl acetate fraction insert ignore into journalissuearticles values(EF); of the methanol leaf extract of Phyllanthus muellerianus. The structures of the isolated compounds were elucidated by 1H-NMR and by mass spectroscopy. The effects of the isolated compounds on phagocytic competence of macrophages and neutrophils using in vitro models were evaluated. Compounds 1 and 2 significantly insert ignore into journalissuearticles values(p<0.05); increased both phagocytic ability and intracellular killing capacity of neutrophils. This study established that the tannin glycosides stimulated cell-mediated innate immunity by increasing the phagocytic function of neutrophils and thus may be useful in both the clinical and prophylactic treatment of intracellular microbial infections.
Keywords : Glycosides, Cell mediated, Immunity, Phygocytosis, Neutriphills, Macrophages

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