Antibiofilm Effect of Moringa oleifera Leaf Extract Against Staphylococcus aureus, Cytotoxicity, Biochemical aspects, Anti-Inflammatory potential, and Interference on the Activity of Antimicrobial Drugs

Authors : Lorena KİMBERLY SİLVA ALCÂNTARA, Luiz Felipe CARREİRO MACHADO, Isabela PENNA CERAVOLO, Renan MARTİNS, Marcus Vinícius DİAS SOUZA
Pages : 241-254
Doi:10.55262/fabadeczacilik.1213447
View : 126 | Download : 55
Publication Date : 2023-08-01
Article Type : Research Paper
Abstract :Abstract: There is a growing technical difficulty to treat infectious diseases due to molecular mechanisms of resistance to antimicrobial drugs, such as biofilm formation. Here we provide evidence of the antimicrobial potential of the hydroethanolic extract of Moringa oleifera leaves, traditionally used to treat disorders like cardiovascular and endocrine diseases, on clinical isolates of Staphylococcus aureus. The extract was chemically characterized using classic techniques and by ultra performance liquid chromatography insert ignore into journalissuearticles values(UPLC);. We carried out minimum inhibitory concentration tests, as well as minimal bactericidal concentration and minimal biofilm eradication concentration tests. Moreover, we tested the anti-inflammatory potential and assessed the toxicity of the extract on buffalo green monkey insert ignore into journalissuearticles values(BGM); cells. We also investigated the effects of combining the extract to clinically relevant antimicrobial drugs insert ignore into journalissuearticles values(i.e., synergistic or antagonistic interactions);. The extract was active at 8 g/mL and 16 g/mL for planktonic cells and biofilms, respectively. Its anti-inflammatory potential was confirmed, and it lacked cytotoxicity. No significant interference of the extract on antimicrobial drugs was observed. Flavonoids, tannins, proteins, carbohydrates and vitamin C were detected in the extract. Our data open doors for further studies with isolated molecules of the extract in order to conduct in vivo antimicrobial tests.
Keywords : Moringa oleifera, Staphylococcus aureus, antimicrobial, biofilms

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