Spectrophotometric Determination of Mesalazine in Pure Form and Pharmaceutical Formulations by Diazotization and Coupling With 2,7-Dihydroxynaphthalene As a New Coupling Agent

Authors : Aws MASEER, Moath NAJEM

Pages : 195-203

Doi:10.52794/hujpharm.1129852

View : 69 | Download : 73

Publication Date : 2023-09-01

Article Type : Research Paper

Abstract :A simple, specific and sensitive spectrophotometric method is presented for the determination of Mesalazine insert ignore into journalissuearticles values(MZN); based on diazotization in an acidic medium through reaction with sodium nitrite insert ignore into journalissuearticles values(NaNO2); to form a diazonium salt that coupling reaction in a base medium with 2,7-Dihydroxynaphthalene insert ignore into journalissuearticles values(DHNP); to form an azo dye. The dye intensity was measured at 501 nm after optimization of the experimental parameters. Beer’s law was applied to the proposed method, valid within a concentration range of 0.25–10 μg.mL-1, and the linear regression was R2 = 0.9974. The limit of detection insert ignore into journalissuearticles values(LOD);, the limit of quantification insert ignore into journalissuearticles values(LOQ);, the molar absorptivity coefficient were 0.074 μg.mL-1,0.249 μg.mL-1 and 1.85x104 L.mol−1.cm−1, respectively. Sandell\`s sensitivity was 0.0082μg.cm-2. There is no interference from excipients found in the tablet. The data were statistically compared with the British Pharmacopeia using two statistical methods insert ignore into journalissuearticles values(t-test and F-test);.
Keywords : Mesalazine MZN, 7 Dihydroxynaphthalene DHNP, Diazotization, Coupling Reaction