Identification of Vibrio parahaemolyticus by PCR Method in Fish and Water Samples from Tigris River, Turkey

Authors : Filiz ÖZCAN, Neval Berrin ARSERİM, Aydın KETANİ

Pages : 191-195

Doi:10.31196/huvfd.1009028

View : 21 | Download : 6

Publication Date : 2021-12-12

Article Type : Research Paper

Abstract :Climate change has caused increasing Vibrio parahaemolyticus infections in areas unaffected by this pathogen. Water temperature is an important ecological factor. It is consequently of high importance to follow V. parahaemolyticus in aquatic environments and foods. The aim of this work was to detect the presence of V. parahaemolyticus in Tigris River from eight different sites namely Malabadi Bridge, Fiskaya, Ongözlü Bridge, Bağıvar, Pamukçay, Ambarçay, Seyrantepe and Silvan. Total 480 samples of water and fish were used for V. parahaemolyticus detection. Thiosulphatecitrate‐bile salt sucrose (TCBS) Agar was employed for bacteria isolation for 24 h at 37◦C. The dominant uniform bacterial colonies were purified by streaking onto the TCBS agar plates three times. Identification of this pathogen was made by PCR. A total of 480 water and fish samples were analyzed. According to the results, V. parahaemolyticus was detected in 40 (6.6%) fish samples taken from the Seyrantepe region.
Keywords : pcr, Vibrio parahaemolyticus, Fish, Water