Three Previously Unseen Genotypes Detected in IGFBP - 3 Gene in Buffalo Breed

Authors : Yusuf ÖZŞENSOY, İnanc BARAL

Pages : 35-40

Doi:10.31196/huvfd.1245427

View : 42 | Download : 44

Publication Date : 2023-06-22

Article Type : Research Paper

Abstract :Anatolian water buffalo breed is Turkey’s sole water buffalo breed, and their numbers steadily increased with the national “Water Buffalo Breeding by Breeders Project”. This study aimed to investigate of the gene region polymorphisms insert ignore into journalissuearticles values(Intron-2, Exon-2/Intron-3, Exon-3); of the meat-yield-related Insulin-like Growth Factor Binding Protein-3 insert ignore into journalissuearticles values(IGFBP-3); gene in Anatolian water buffaloes by TaqI, HaeIII, and MspI restriction endonucleases. The phenol/chloroform method was used for DNA isolation from 151 blood samples, and extracted DNAs were amplified by touchdown PCR using specific primers. Amplified PCR products were digested with restriction endonucleases insert ignore into journalissuearticles values(REs); and separated in 3% agarose gel electrophoresis insert ignore into journalissuearticles values(AGE);, then genotypes were determined. Results revealed two genotypes [AA insert ignore into journalissuearticles values(98.68%); and AC insert ignore into journalissuearticles values(1.32%);] and two alleles [A insert ignore into journalissuearticles values(0.99); and C insert ignore into journalissuearticles values(0.01);] for the Exon-2 to Intron-3 region from HaeIII digestion. TaqI digestion of the Intron-2 region revealed three genotypes [AA insert ignore into journalissuearticles values(7.94%);, AB insert ignore into journalissuearticles values(3.97%);, and BB insert ignore into journalissuearticles values(88.10%);] and two alleles [A insert ignore into journalissuearticles values(0.10); and B insert ignore into journalissuearticles values(0.90);]. MspI digestion of the Exon-3 region revealed only the AA genotype and A allele, thus revealing monomorphism. Overall, HaeIII digestion revealed insignificant polymorphism insert ignore into journalissuearticles values(P>0.05);, and TaqI digestion revealed significant polymorphism insert ignore into journalissuearticles values(P<0.001); for their respective regions. Gene polymorphisms of these regions were investigated for the first time in Anatolian water buffaloes. Additionally, three novel genotypes for the IGFBP-3 gene insert ignore into journalissuearticles values(one from HaeIII and two from TaqI); were determined for the first time. The novel B allele from TaqI digestion was observed to have a substantial frequency.
Keywords : Anadolu Mandası, HaeIII, IGFBP 3, PZR RFLP, Sivas, TaqI