The Expression Dynamics of Key Immune-Related Genes in Response to Mannheimia Haemolytica in Sheep Alveolar Macrophages In Vitro

Authors : Saif Adil Abbood ALJANABI, Mehmet Ulaş ÇINAR, Ghulam Asghar SAJİD, Ahmed Qasim Naji NAJİ, Md Mahmodul Hasan SOHEL

Pages : 1-13

Doi:10.51970/jasp.1255946

View : 26 | Download : 30

Publication Date : 2023-06-29

Article Type : Research Paper

Abstract :Alveolar macrophages insert ignore into journalissuearticles values(AMs); respond to these infections as the first line of defense and trigger the lung\`s immune response. Knowing the expression dynamics of key immune-related genes in alveolar macrophages against M. haemolytica challenge will help deepen the understanding of disease immunopathogenesis. For this purpose, first time an in vitro obtained from bronchoalveolar lavage insert ignore into journalissuearticles values(BAL); fluid and treated with three doses insert ignore into journalissuearticles values(T1: 1800, T2: 2700, T3: 5400 CFU); of M. haemolytica inoculum, while keeping one untreated as a control. Then, total RNA was extracted, and cDNA was synthesized. The real-time quantification data indicated that the expression of IL-1β, IL-6, IL-8, IL-10, and NF-κB genes were significantly changed as compared to control. Our study revealed that the exposure to M. haemolytica stimulates the immune response in the sheep alveolar macrophages in a dose and time-dependent manner.
Keywords : Alveolar makrofaj, Bağışıklıkla ilişkili genler, Gen ifadesi, M haemolytica, pnömoni