Determination of Antioxidant, Antimicrobial, and Antiproliferative Activities of Onobrychis argyrea subsp. argyrea Extracts

Authors : Sevgi ALTIN, Cemalettin ALP, Ekrem KÖKSAL, Sümeyye AKYÜZ

Pages : 1134-1141

Doi:10.21597/jist.1240818

View : 77 | Download : 68

Publication Date : 2023-06-01

Article Type : Research Paper

Abstract :Plants belonging to the Fabaceae family have great importance in animal nutrition and the protection of human health insert ignore into journalissuearticles values(antidiabetic, anticarcinogenic, antioxidant, anti-inflammatory, and cardiovascular);. Onobrychis, known as a potent antioxidant, anticarcinogenic and antimicrobial agent, its high phenolic substance is one of the important genera of this family. In this study, chloroform insert ignore into journalissuearticles values(OAC);, ethanol insert ignore into journalissuearticles values(OAE);, and water insert ignore into journalissuearticles values(OAW); extract of Onobrychis argyrea subsp. argyrea insert ignore into journalissuearticles values(O. argyrea); specie was prepared; and the antiproliferative, antimicrobial, and antioxidant activities were investigated. The antiproliferative effects of the extracts were evaluated on different cancer cell lines by XTT colourimetric method. OAE demonstrated the most excellent efficacy against all cell lines, according to the findings. MCF-7 83.71%, A549 92.14%, and HT-29 72.24% were all inhibited. The antimicrobial activity of OAE and OAW extracts against Staphylococcus aureus, Escherichia coli, and Streptococcus pneumoniae was determined using the disc-diffusion technique. Finally, antioxidant power was determined using DPPH and FRAP methods. In the DPPH research, OAE and OAW extracts showed the highest levels of inhibition insert ignore into journalissuearticles values(IC50: 34.12±0.2 and 21.58±0.12, respectively);. OAE insert ignore into journalissuearticles values(257.95±1.40); and OAW insert ignore into journalissuearticles values(282.14±0.83); extracts showed high activity with similar results in the FRAP method. Total phenolic content was determined as 226.15±1.89 mg GAE/g for OAE and 146.52±0.71 mg GAE/g for OAW, respectively. These results show that the species is an essential source of antioxidants. These studies are the first data for Onobrychis argyrea subsp. argyrea. All these results show that the plant has various biological activities and rich secondary metabolites.
Keywords : Onobrychis argyrea subsp argyrea, antioksidan, antiproliferatif, antimikrobial

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