Inhibitor Effects of Some Artificial Tears Drops on Human Carbonic Anhydrase Enzyme-II (hCA-II)

Authors : Büşra ÇALIŞKAN, Mine AKSOY

Pages : 941-947

Doi:10.21597/jist.1253344

View : 66 | Download : 34

Publication Date : 2023-06-01

Article Type : Research Paper

Abstract :Carbonic anhydrases insert ignore into journalissuearticles values(CAs); are a metalloenzyme that contains zinc ions in their active sites, and they reversibly catalyze the reactions of hydration of CO2 and dehydration of HCO3-in the living. In this study, human carbonic anhydrase II insert ignore into journalissuearticles values(hCAII); isoenzyme was purified from human erythrocytes by affinity column insert ignore into journalissuearticles values(Sepharose 4B-L-tyrosine-sulfanilamide);. The inhibitory effect of artificial tear drops on hCAII isoenzyme was investigated. IC50 values were calculated as 1.01 µL for sodium hyaluronate, 140.06 µL for polyvinyl alcohol+povidone, and 49.51 for polyethylene glycol+propylene glycol. The Ki value for sodium hyaluronate was found as 0.339±0.121 mM. According to these results, sodium hyaluronate was the most effective inhibitor in terms of application volume insert ignore into journalissuearticles values(microliter); among the three drugs examined.
Keywords : İnsan karbonik anhidraz II, Suni gözyaşı damlaları, İnhibisyon

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