The Preparation, Bioactivity and HPLC-UV Contents Analysis of Antibacterial Chewable Bars

Authors : Ayşegül Hoş, Ayşe İnci, Ayşe Çalış, Ebrar Oktay, Dilara Demirel, Gülpembe İmrak, Ozan Emre Eyupoglu

Pages : 448-458

Doi:10.21597/jist.1595057

View : 91 | Download : 56

Publication Date : 2025-06-01

Article Type : Research Paper

Abstract :This study describes the development of antibacterial eco-friendly disposable chewable bar in order to provide convenience to the user in cases where hygiene methods are difficult to apply or there is not enough time and materials for application. Firstly, essential oils and methanolic extracts of clove, lavender and pomegranate fruit peel were obtained. Disc diffusion method was used to find out the antibacterial activity of each extracts and essential oils against Streptococcus mutans, Streptococcus salivarius, Streptococcus mitis, Staphylococcus aureus, Staphylococcus epidermidis. Analyses of HPLC-UV were carried out by using the HPLC Agilent-1100 modular compact system. Cinnamic acid, Caffeic acid, Punicalagin, Kaempferol-3-O-rutinoside, Quercetin-3-O-hexoside, and Catechin were detected in all methanolic extracts. Next, antibacterial chewable bars, which were made of beeswax, mastic gum, extracts and essential oils of lavender, clove, pomegranate fruit peel, were prepared. Then, the antibacterial activity of antibacterial chewable bars was determined against human saliva, S. mitis, S. aureus, S. epidermidis. The chewable bar 1, which contains beeswax and mastic gum, showed 71-90% antibacterial activity against S. aureus, S. mitis and S. epidermidis. The chewable bar 2, which includes beeswax, mastic gum, extracts and essential oils, showed 100% antibacterial activity against S. aureus, S. mitis and S. epidermidis. Also, chewable bar 2 has 99% antibacterial activity against human saliva.The current research is the first research about the preparation of antibacterial chewable bar and the determination of the antibacterial activity of it. It is needed further research to discover the side effects of this antibacterial chewable bar before using for human.
Keywords : Ağız bakımı, antibakteriyel aktivite, disk difüzyon yöntem, plaka sayımı, biyobozunur malzeme

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