Software Development with the R Programming Language for Studying Tyrosinase Enzyme Kinetics in Giresun Chanterelle Mushrooms

Authors : Evren Altıok, Maya Mahfuz

Pages : 2069-2081

Doi:10.31466/kfbd.1518620

View : 29 | Download : 44

Publication Date : 2024-12-15

Article Type : Research Paper

Abstract :In this study, a software application for enzyme kinetics analysis was developed using the R programming language. The software was designed utilizing the kinetic measurement values of tyrosinase enzyme from Giresun chanterelle (Cantharellus cibarius) mushrooms. Detailed step-by-step documentation of the software development process is provided. The program includes statistical evaluations and applies the Michaelis-Menten enzyme kinetics model to describe the enzyme kinetics of Giresun chanterelle. Initial reaction rate values were determined with a high correlation and regression coefficient (R² > 0.95). The software plotted rate values against substrate concentration, yielding Km and Vmax values of 0.25 mM and 0.0016 mM/s, respectively. These values were used to plot the theoretical curve, and its correlation with experimental data was statistically analyzed. The results demonstrate that the developed software can rapidly analyze data with minimal errors. As an open-access and extensible tool, the program can be effectively utilized and further developed for studies on enzyme activation or inhibition.
Keywords : R programlama, Enzim Kinetiği, Michaelis-Menten denklemi, Giresun kantarel mantarı, Tirozinaz