Antimicrobial Activity and Mode of Action of Phellinus hartigii Extract Investigated on Multidrug-Resistant Staphylococcus aureus Using Fourier-Transform Infrared Spectroscopy

Authors : Kaan Akçay, Kübra Tekşen, Umay Merve Şenturan, Eda Altınöz, Dilşad Özerkan, İlgaz Akata, Ergin Murat Altuner

Pages : 409-421

Doi:10.17475/kastorman.1845436

View : 77 | Download : 191

Publication Date : 2025-12-25

Article Type : Research Paper

Abstract :Aim of study: Macrofungi have garnered attention as promising reservoirs for novel antibiotics. Phellinus hartigii is known to have antimicrobial properties against various microorganisms. This study aims to investigate the in vitro mode of action of the ethanol extract of P. hartigii (PH-EtOH) against multidrug-resistant (MDR) Staphylococcus aureus using Fourier-transform infrared (FTIR) spectroscopy. Material and method: The composition of the extract was determined by GC-MS analysis, and its antimicrobial activity was evaluated through MIC and MBC tests. The effect of the extract was further analysed using FTIR spectroscopy. Main results: The PH-EtOH extract demonstrated antibacterial activity with a MIC of 53 µg/mL and an MBC of 420 µg/mL. The major constituents of PH-EtOH were ethyl oleate, linoleic acid ethyl ester, and hexadecanoic acid ethyl ester. FTIR spectra showed changes mainly in proteins and nucleic acids. Research highlights: The results of this study provide a starting point for further research into the use of P. hartigii ethanol extract as an antimicrobial agent against MDR S. aureus.
Keywords : Phellinus hartigii, Staphylococcus aureus, Antimikrobiyal Aktivite, Etki Mekanizması, FTIR

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