The Effects of In Ovo Administered Monosodium Glutamate on the Embryonic Development of Skeletal Muscle in Chickens

Authors : Ferhan BÖLÜKBAŞ
Pages : 47-56
Doi:10.30607/kvj.1223940
View : 38 | Download : 9
Publication Date : 2023-03-31
Article Type : Research Paper
Abstract :The aim of this study was to investigate the effects of monosodium glutamate insert ignore into journalissuearticles values(MSG); in ovo administration on embryonic skeletal muscle development in chicken embryos, using histological, histopathological, and immunohistochemical methods. In this study, a total of 410 fertilized chicken eggs were used and were divided into five group as control, vehicle control, low-dose group insert ignore into journalissuearticles values(0.12 mg/g egg MSG);, medium-dose group insert ignore into journalissuearticles values(0.6 mg/g egg MSG);, and high-dose group insert ignore into journalissuearticles values(1.2 mg/g egg MSG);. At incubation days 18 and 21, eggs from each group were opened and six live embryos were obtained. The embryos were sacrificed by decapitation, and skeletal muscle tissue samples insert ignore into journalissuearticles values(musculus fibularis longus and musculus sternocoracoideus pectoralis); were obtained. Sections were stained with hematoxylin-eosin. Moreover, caspase-3 reactivity was determined using the immunohistochemical method. Muscle development was delayed in the MSG groups and the number of caspase3-positive cells was higher insert ignore into journalissuearticles values(p < 0.05); than in the controls. Histopathological examinations revealed degenerative and necrotic changes in the skeletal muscle. Muscle degeneration insert ignore into journalissuearticles values(edema, muscle fiber degeneration, Zenker’s necrosis, and mononuclear cell infiltrations); were observed in all groups, except for the control groups. It was concluded that MSG could adversely affect the development of the skeletal muscle.
Keywords : Embriyotoksisite, İskelet kası, Kaspaz 3, Monosodyum glutamate

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