Effect of Alpha Lipoic Acid on Polychlorinated Biphenyl (Aroclor 1254)-Induced Nephrotoxicity in Rats

Authors : Mustafa Yildiz

Pages : 504-512

Doi:10.30607/kvj.1364111

View : 46 | Download : 63

Publication Date : 2023-12-31

Article Type : Research Paper

Abstract :The present study aimed to determine the morphological changes in rat kidneys exposed to PCB (Aroclor 1254) and to reveal the effects of ALA on those changes. Rats were randomly divided into four groups: Control, ALA, PCB and ALA+PCB. The applications of ALA and PCB continued for thirty days. At the end of the experiment, the tissue samples were taken from the animals. Crossmon’s triple staining method was applied to kidney sections for histopathological and histomorphometric examinations. In addition, the periodic acid-Schiff (PAS) reaction was examined in tissue sections. Histopathologically, it was determined that glomerular degeneration and connective tissue densities in the kidney significantly increased in PCB group compared to Control group. Besides, it was found that glomerular degeneration intensity significantly decreased in ALA+PCB group compared to PCB group. It was detected that the diameters of the renal corpuscle, proximal tubule, ascending limb of Henle’s loop and collecting duct in the kidney significantly decreased in PCB group compared to Control group. However, these parameters significantly increased in ALA+PCB group compared to PCB group. While the density of glomerular mesangial matrix and glycogen density in proximal tubule epithelium significantly increased in PCB group compared to Control group, these parameters significantly decreased in ALA+PCB group compared to PCB group. As a result, PCB may adversely affect renal functions by causing disorders in kidney histology. In addition, the use of ALA against PCB exposure may contribute to the preservation of kidney tissue.
Keywords : Alfa lipoik asit, Aroklor 1254, Böbrek, Histoloji, Sıçan