Isolation and Molecular Characterization of Colistin-Resistant Escherichia coli in Raw Meat Samples

Authors : Mukaddes Barel

Pages : 48-54

Doi:10.30607/kvj.1414055

View : 92 | Download : 100

Publication Date : 2024-03-31

Article Type : Research Paper

Abstract :Antimicrobial resistance occurring in a wide variety of Gram negative bacteria is considered an ongoing public health threat. For this reason, unconscious use of antimicrobials reveals new resistance mechanisms, and these resistance mechanisms are spreading globally. Due to these reasons, our current study aimed to isolate colistin-resistant Escherichia coli in raw meat samples and molecular characterization of resistance genes. For this purpose, the trpA gene was detected in 90 (75%) of the E. coli isolates obtained from 300 samples. mcr-1 gene was found in 8 (8.8%) of the isolates confirmed as E. coli. However, as a result of the PCR analysis, O45:H2, O103:H2, O121:H19, O145:H28, O26:H11, O111:H8 E. coli serogroups and virulence genes were not found in any isolate. Additionally, isolates 7 (7.7%), 5 (5.5%), 29 (32.2%), 23 (25.5%), 8 (8.8%), 10 (9%), 2 (2.2%) and 6 (6.6%). It was found to be acid resistant to tetracycline, erythromycin, gentamicin, azithromycin, imipenem, ampicillin and nalidixic acid, respectively.
Keywords : Antimikrobiyal Dirençlilik, Escherichia coli, Kolistin