Commercial Lavender (Lavandula Angustifolia) Oil’s Effects On Buck Spermatozoa During Cryopreservation

Authors : Nail Tekin Önder, Oğuzhan Şahin, Muhammet Can Kılıç, Seda Gökdemir, Serpil Aygörmez, Taygun Gökdemir, Mustafa Reha Coşkun, Savaş Yıldız, Yavuz Öztürkler

Pages : 75-81

View : 41 | Download : 62

Publication Date : 2025-03-28

Article Type : Research Paper

Abstract :The objective of this study was to assess the impact of lavender oil preparation on the spermatological data, oxidant-antioxidant parameters, and antimicrobial properties of frozen buck semen due to its antioxidant and antibacterial characteristics. To conduct a comprehensive analysis of these impacts, the study established nine experimental groups. Three control groups designed as; a negative control group, a group treated with 1000 IU/mL penicillin and 1000 µg/mL streptomycin (C1), and a group treated with 500 IU/mL penicillin and 500 µg/mL streptomycin (C2). Additionally, groups were created by adding lavender oil to the base diluent. Commercial lavender oil was diluted at a ratio of 10-4. We then formed four groups, each receiving different amounts of this diluted oil: 10 µL/mL, 15 µL/mL, 20 µL/mL, and 25 µL/mL. Simultaneously, two distinct groups were formed by using dosages of 20 µL/mL and 25 µL/mL by adding to the C2 group. The study revealed that lavender oil had protective effects on motility, plasma membrane integrity, and acrosome integrity. Furthermore, lavender oil\\\'s antioxidant properties positively affect frozen-thawed semen, and its antibacterial impact was shown when used in conjunction with the C2 group.
Keywords : Lavanta esansiyel yağı, teke sperması, antioksidan, kriyoprezervasyon, lavandula angustifolia