The Effect of Carob Aqueous Extract on Oxidative Stress, Proinflammatory Cytokines, Glucose and Lipid Concentrations in the Blood of Diabetic Rats.

Authors : Mehmet Talha Avşar, Abdullah Eryavuz
Pages : 192-199
Doi:10.30607/kvj.1591051
View : 77 | Download : 109
Publication Date : 2025-10-03
Article Type : Research Paper
Abstract :Herbal treatments offer potential benefits in controlling blood glucose levels and preventing diabetes-related complications. This study was conducted to determine the effects of the application of carob fruit aqueous extract prepared by ultrasound-assisted extraction (UAE) method on oxidative stress, glycemic level, proinflammatory cytokines and lipid profile levels in the blood of rats with experimental diabetes induced by streptozotocin-nicotinamide (STZ-NA) model. Forty male Wistar Albino (200-250 g live weight) animals used in the study were divided into four groups as normal control, diabetic control, normal group given 200 mg.kg-1 carob aqueous extract and diabetic group given 200 mg.kg-1 carob aqueous extract. At the end of the 21-day study, plasma glucose, hemoglobin A1C (HbA1c), insulin, homeostatic model assessment of insulin resistance (HOMA-IR), malondialdehyde (MDA), reduced glutathione (GSH), proinflammatory cytokines interleukin 1-beta (IL-1β), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), total cholesterol, triglyceride, leptin and 25-hydroxy vitamin D levels were measured in blood samples taken from all animals. Carob aqueous extract treatment did not cause a significant decrease in high glucose, HbA1c and HOMA-IR values caused by diabetes. The blood insulin levels were not affected by the treatments. Diabetes increased MDA, IL-6 and triglyceride levels (p<0.05), while carob aqueous extract treatment to diabetic animals did not affect these parameters. However, carob aqueous extract treatment to diabetic animals increased GSH levels (p<0.05). The treatments had no effect on IL-1β, TNF-α, total cholesterol and leptin levels. Conclusively, the carob fruit aqueous extract treatment did not have an effect on controlling diabetes-related hyperglycemia.
Keywords : Diyabet, Keçiboynuzu sulu ekstraktı, oksidatif stres, hiperglisemi, dislipidemi

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