Effect of Chemical Castration Using High Osmolarity Solutions on Spermatological Parameters in Rats

Authors : Şeyma Özer Kaya, İbrahim Canpolat, Hasan Akın
Pages : 236-243
Doi:10.30607/kvj.1674274
View : 48 | Download : 89
Publication Date : 2025-10-03
Article Type : Research Paper
Abstract :In our study, the changes in spermatological parameters in rats subjected to chemical castration with high osmolarity solutions were investigated. In the study, they were divided into 8 groups, with 8 rats in each group, and a total of 64 Wistar albino male rats were used. No chemicals were applied to the rats in the control group. Rats in the second group were sterilized surgically. 0.9% physiological saline was administered to the 3rd group, 0.9% physiological saline and 10% calcium chloride to the 4th group, 20% mannitol to the 5th group, 20% mannitol and 10% calcium chloride to the 6th group, 20% dextrose to the 7th group, 20% dextrose and 10% calcium chloride solutions were administered intratesticularly to the 8th group at a dose of 0.1 ml/100gr. Andrological findings compared to the control group; it was determined that there was a significant difference in sperm motility and sperm density (p<0.001) in groups 3, 5, and 7. However, it was observed that there was no difference in the rate of abnormal spermatozoon. In groups 2, 4, 6, and 8, motility, density, abnormal sperm count and epididymis weights could not be measured because sperm cells could not be collected. In addition, it was determined that the weights of the epididymis and right cauda epididymis in groups 3, 5, 7 decreased compared to the control (p<0.05), while in groups 2, 4, 6, 8, measurements could not be made because tissue samples could not be taken, and the weights of testicles, vesicle seminalis and prostate decreased significantly (p<0.001) compared to the control. In conclusion; it has been concluded that giving high osmolarity solutions in combination with calcium chloride will be much more effective in the intratesticular chemical sterilization process.
Keywords : Kimyasal kastrasyon, spermatolojik parametreler, rat, kalsiyum klorür

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