Spermatological and Biochemical Examination of New Zealand Rabbit Semen After Thawing

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Kocatepe Veteriner Dergisi
Cilt: 18 Sayı: 3
Spermatological and Biochemical Examination of New Zealand Rabbit Semen After Thawing

Spermatological and Biochemical Examination of New Zealand Rabbit Semen After Thawing

Authors : Caner Ozturk, Mustafa Eren Döşeyici, Neşe Hayat Aksoy

Pages : 207-214

Doi:10.30607/kvj.1677781

View : 67 | Download : 56

Publication Date : 2025-10-03

Article Type : Research Paper

Abstract :In the present study, the effects of caffeic acid on rabbit semen were investigated using the antioxidant substance trehalose as a positive control. In the experiment, 4 male rabbits and 1 female rabbit were used. Ejaculates were collected via the artificial vaginal method 2 times a week for 4 weeks. Pooled semen samples were divided into four groups and diluted as follows: basic diluent+6%DMSO+caffeic acid (25µM and 50µM), basic diluent+6%DMSO+trehalose (positive control) (50mM), and basic diluent+6%DMSO (Control). After dilution, the semen samples were aspirated into straws and allowed to equilibrate at +4°C for 1 h. The samples were frozen in liquid nitrogen vapor and stored in liquid nitrogen. The highest value of sperm motility was detected in the 50 mM trehalose group (46.25±1.25%), which was significantly different from that in the control group (35.63±1.99%) (p<0.05). The sperm membrane integrity (SYBR 14/PI) results were determined to be 47.11±0.96%, 49.05±0.85%, and 49.79±1.04% in the 25 µM caffeic acid, 50µM caffeic acid, and 50 mM trehalose groups, respectively, and a significant difference was found between the supplement groups and the control group (39.01±1.21%) (p<0.05). In terms of acrosome integrity (FITC-PNA), the highest value was reached in the trehalose 50 mM group (48.70±1.03%), and a significant difference was observed compared with that in the control group (41.48±0.80%). A significant difference was observed in the total antioxidant capacity and total oxidant level values between the 50 mM trehalose group and the control group (p<0.05). As a result, caffeic acid added to the extender when rabbit semen was frozen had a better protective effect at higher doses.
Keywords : kafeik asit, kriyoprezervasyon, floresan boyama, oksidatif stres, tavşan sperması

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