ENHANCED OSTEOGENIC DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS BY FLEXIBLE β-TCP/PLA BONE GRAFTS WITH SILICATE ADDITIVE

Authors : Günnur ONAK PULAT, Gülşah SUNAL, Ozan KARAMAN

Pages : 770-782

Doi:10.36306/konjes.1198527

View : 29 | Download : 30

Publication Date : 2023-09-01

Article Type : Research Paper

Abstract :In recent years, ceramics, polymers, and composites have been used to develop biologically and mechanically suitable bone scaffolds. β-tricalcium phosphate（β-TCP); is a widely used bioceramic in bone tissue engineering. It shows excellent osteoconductivity, osteoinductivity, and good biocompatibility properties, as its chemical composition is similar to the original chemical structure of bone. Herein, we designed β-TCP-PLA composite scaffolds containing two different concentrations of silicate additives. We aimed to investigate the effect of silicate-additive with varying concentrations insert ignore into journalissuearticles values(0.8% and 1%); on osteogenic differentiation of human bone marrow-derived mesenchymal stem cells insert ignore into journalissuearticles values(hMSCs); seeded flexible bone grafts. The morphological structure of β-TCP-PLA-based bone grafts was assessed by scanning electron microscopy insert ignore into journalissuearticles values(SEM); and the tensile strength of grafts was evaluated. The results showed that scaffolds had porous and flexible structures. hMSCs osteogenic differentiation was evaluated with the alkaline phosphatase insert ignore into journalissuearticles values(ALP); activity and DNA content measurements. Compared with β-TCP-PLA grafts, these designed synthetic flexible bone grafts with 0.8% and 1% silicate-additive significantly promoted hMSCs proliferation and osteogenic differentiation. Moreover, 0.8% silicate-additive β-TCP-PLA grafts showed increased ALP activity. The outcomes of the present study suggest that synthetic flexible bone grafts with silicate-additive might be useful for encouraging the regeneration of bone.
Keywords : kemik doku mühendisliği, β TCP PLA kemik greftleri, silikat, osteogenez