Verticillium dahliae ile İnokule Edilen Yonca Bitkisinden (Medicago sativa cv. Vertus) Elde Edilen Fenilalanin Amonia Lyaz Enziminin Kademeli Olarak Saflaştırılması

Authors : Murat DİKİLİTAS

Pages : 19-22

Doi:10.18016/ksujns.60327

View : 20 | Download : 13

Publication Date : 2012-12-31

Article Type : Other Papers

Abstract :  ABSTRACT: An increased activity in phenylalanine ammonia lyase (EC 4.3.1.24, PAL) enzyme in lucerne seedlings ( Medicago sativa L. cv. Vertus) following inoculation with Verticillium dahliae was measured after 48 h of inoculation.  Purifications of PAL with 0-50% (NH 4 ) 2 SO 4 precipitation and Sephadex G-100 gel filtration resulted in 1.75 and 1.97 fold increases with 75 and 60 % recoveries, respectively.  This study showed that the purification of the enzyme clearly resulted in significant differences in respect to activities between fractions.  This property could be important for further characterization of pathogen-related proteins.
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