Relationship Between ISX Gene Expression and Cancer Stem Cell Markers in Hepatocellular Carcinoma Cells Exposed to Microenvironmental Changes

Authors : Hüseyin Şimşek, Zeynep Gelirli, Gamze Demirel, Zeynep Akbulut

Pages : 6-12

Doi:10.35514/mtd.2025.121

View : 23 | Download : 59

Publication Date : 2025-04-29

Article Type : Research Paper

Abstract :Aim: This study aimed to demonstrate the homeobox (ISX) protein, which is a newly identified proto-oncogene and known to be associated with hypervitaminosis A disease at the level of ISX gene expression by culturing hepatocellular carcinoma (HCC) cell line in different extracellular matrix media and to show the effect of culture media differences on cancer stem cell markers. Material and Methods: ISX expression was 4 times higher in Matrigel culture and 2 times higher in type I collagen culture compared to the control group. Expression of the Oct-4 antibody was significantly higher in type I collagen culture medium compared to the control group (p<0.05). Nanog antibody showed a significant decrease in HepG2 cells in Matrigel culture compared to the control (p<0.05), while Ov-6 and AFP expression was highest in type I collagen, but no statistically significant difference was found (p>0.05). Results: ISX expression was detected 4-fold higher in the Matrigel and 2-fold higher in the type I collagen culture than the control group. The Oct-4 antibody is significantly higher in the type I collagen culture (p<0.05) and the Nanog antibody showed a significant decrease in the Matrigel culture than the control group cells (p<0.05). Ov-6 and AFP antibodies are the highest type I collagen but there are no statistical significance was found (p>0.05). Conclusion: It is thought that the results obtained from this study may help further studies on the mechanisms of liver cancer and the development of treatment options that will affect the behavior of cancer stem cells.
Keywords : Hepatoselüler karsinom, A vitamini, ISX geni, Tip1 kollajen, Matrigel