Effects of Colchicine and Trypsin Treatments on Metaphase Chromosome Spreading from Larvae and Different Tissues of Adult Female and Male Nile Tilapia

Authors : Burak Evren İnanan

Pages : 113-118

Doi:10.58626/menba.1538957

View : 43 | Download : 69

Publication Date : 2024-12-28

Article Type : Research Paper

Abstract :Assessment of results of different biotechnological methods in aquaculture such as ploidy, gynogenesis, and androgenesis are needed cytogenetical techniques. Achieving well-distributed metaphase chromosome spreads is a preeminent step for cytogenetical techniques. The aim of this study was to evaluate the effects of colchicine and trypsin treatments on the yield of countable metaphase chromosome spreads. With this aim, chromosome preparations from gill, spleen, kidney, and caudal fin tissues of adult females and males Nile tilapia, and also whole body of their larvae were carried out. Based on the previous studies reviewed so far, this is the first systematic and extensive study indicated that the effects of colchicine and trypsin on metaphase chromosome spreads regarding gender differences and larvae. In the chromosome preparations, it was designated four treatments as “Trypsin (-) Colchicine (-)”, “Trypsin (-) Colchicine (+)”, “Trypsin (+) Colchicine (-)”, and “Trypsin (+) Colchicine (+)”. Effects of colchicine and trypsin in terms of their usage in chromosome preparations were evaluated by determining the frequencies of metaphase cells. For this purpose, metaphase index were used to reveal differences in detected metaphase cells among the treatments. The highest metaphase index values were calculated in the Trypsin (+) Colchicine (+) treatment group of all tissues. Also, the scores of metaphase index found in spleen and kidney tissues were greater than those found in gill and fin tissues. The present study has also shown that metaphase chromosome spreads could be obtained without colchicine or trypsin treatments.
Keywords : Metfaz dağılımı, Kromozom, Kolşisin, Tripsin, Nile Tilapyası (Oreochromis niloticus)