Isolation, identification and characterization of antagonistic and plant growth promoting mechanisms of Pseudomonas spp. isolated from forest soils

Authors : Yusuf Gümüş, Soner Soylu, Aysun Uysal, Merve Oğuz, Emine Mine Soylu, Şener Kurt

Pages : 840-853

Doi:10.37908/mkutbd.1742348

View : 88 | Download : 231

Publication Date : 2025-12-31

Article Type : Research Paper

Abstract :This research focused on evaluating the plant growth-promoting traits and biocontrol capabilities of Pseudomonas strains isolated from soil samples obtained from two forested regions in Hatay Province. After isolation, the pathogenicity of 16 different Pseudomonas isolates was evaluated using the hypersensitivity reaction (HR) and soft rot assay. Non-pathogenic isolates were identified to species level by MALDI-TOF MS analysis. The selected strains were analysed under laboratory conditions for their plant-promoting and pathogen-suppressive properties, including production of siderophores and proteases, phosphate solubilisation and synthesis of hydrogen cyanide (HCN) and ammonia (NH₃). In particular, P. putida FS12 exhibited high siderophore production, P. extremorientalis FS3 showed a strong ability to dissolve phosphate and P. mandelii FS13 was characterised by strong HCN and NH₃ production. Among the isolates, P. brassicacearum FS16 showed a broad spectrum of biocontrol potential combining several useful mechanisms. These results show that microbial diversity in forest soil is a valuable reservoir for agriculturally useful compounds. The identified Pseudomonas strains are proposed as candidates for the development of biofertilisers or biopesticides, and have the potential to offer environmentally friendly alternatives for sustainable agriculture.
Keywords : Orman toprağı, Pseudomonas, Biyolojik mücadele, Biyogübre, Bitki gelişimini teşvik eden bakteri

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