Effect of Electroporation on Antiproliferative Activity of Rheum Ribes and Hypericum Perforatum Extracts in Lung Cancer Cells

Authors : Mehmet Eşref Alkış, Fuat Yetişsin

Pages : 1-12

Doi:10.71255/maunsbd.1634531

View : 38 | Download : 101

Publication Date : 2025-12-29

Article Type : Research Paper

Abstract :The purpose of this study is to investigate the phenolic substance contents of Hypericum perforatum and Rheum ribes plants and their cytotoxicity on lung cancer cells (A549) and to reveal the effects of electroporation (EP) on the antiproliferative activities of these plants. Antiproliferative activities of plant extracts were determined in A549 cells, and their biocompatibility was evaluated in L-929 fibroblast cells through MTT assay. In electrochemotherapy (extracts+EP) applications of A549 cancer cells, eight square wave electrical pulse sequences with an intensity of 800V/cm were used with various doses of plant extracts. Both plant extracts were observed to be abundant in phenolic compounds and exhibited almost no cytotoxic effects, with IC50 values higher than 1000µg/mL for L-929 fibroblast cells. However, A549 cancer cells showed very good sensitivity to the cytotoxic activities of Rheum ribes and Hypericum perforatum extracts, with IC50 values of 297.32 and 241.10 µg/mL, respectively. The cytotoxic activity of both plant extracts increased considerably with EP, and cell viability percentages of extract+EP groups were observed to decrease significantly compared to extract-only groups (p<0.05). Our findings reveal that Rheum ribes and Hypericum perforatum extracts have anticancer potential and may be promising for lung cancer if used in combination with EP.
Keywords : Hypericum perforatum, Rheum ribes, Akciğer kanseri, Sitotoksisite, Elektroporasyon

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