Ten-Year Recurrence Outcomes in Early Stage and Early Onset Colon Cancer and the Expression Profiles of MicroRNAs and Cancer Stem Cell Markers in These Tumors

Authors : Secil AK AKSOY, Berrin TUNCA, Tuncay YILMAZLAR, Özgen IŞIK, Ersin ÖZTÜRK, Melis MUTLU, Çağla TEKİN, Baris GULCU, Nesrin UGRAS, Ömer YERCİ, Gülşah ÇEÇENER, Ünal EGELİ

Pages : 16-23

Doi:10.26453/otjhs.1074644

View : 15 | Download : 8

Publication Date : 2023-03-05

Article Type : Research Paper

Abstract :Objective: We hypothesized that microRNAs insert ignore into journalissuearticles values(miRNAs); might be involved in tumor development by critically regulating cancer stem cell insert ignore into journalissuearticles values(CSC); markers in the early stages of colon cancer insert ignore into journalissuearticles values(eCC);. This study aimed to determine the expression profiles of miRNAs in CSC-positive eCC patients and examine their associations with recurrence. Materials and Methods: We analyzed CD133, LGR5 and SOX2 expression profiles to determine CSC status in 30 eCC specimens. Then, using the results of RT2 miRNA PCR custom arrays, we evaluated the expression profiles of 38 miRNAs in CSC-positive eCC patients. Results: Recurrence occurred in 5 patients within ten years after surgery. We determined down-regulation of miR-125b and up-regulation of miR-135b were significant in CSC-positive eCC patients insert ignore into journalissuearticles values(p=0.021, p=0.001, respectively);. We found that low expression of miR-125b was associated with recurrence in eCC insert ignore into journalissuearticles values(p=0.0022);. Conclusions: We suggest that recurrence might be prevented by increasing the expression of miR-125b in eCC.
Keywords : kanser kök hücresi, kolon kanseri, erken başlangıçlı, erken evre, mikroRNA