DETERMINATION OF THE CHARACTERISTIC ATTRIBUTES OF COTTONSEED PROTEIN CONCENTRATE

Authors : Melike YÜCETEPE, Merve AKALAN, Kamile BAYRAK AKAY, Mehmet Şükrü KARAKUŞ, Asliye KARAASLAN, Bülent BAŞYİĞİT, Mehmet KARAASLAN
Pages : 483-497
Doi:10.15237/gida.GD22115
View : 31 | Download : 9
Publication Date : 2023-04-15
Article Type : Research Paper
Abstract :The current study focused on characteristic attributes of protein concentrate obtained from oil-free cottonseed. For this, the physicochemical properties namely moisture content, water activity, color, flowability, wettability, and protein solubility of cottonseed protein concentrate insert ignore into journalissuearticles values(CSPC); were investigated. Water holding capacity insert ignore into journalissuearticles values(WHC);, oil binding capacity insert ignore into journalissuearticles values(OBC);, foaming capacity, foam stability insert ignore into journalissuearticles values(10 and 30 min);, emulsion activity index insert ignore into journalissuearticles values(EAI); and emulsion stability index insert ignore into journalissuearticles values(ESI); insert ignore into journalissuearticles values(10 and 30 min); of proteins were 2.75 g water/g protein, 2.59 g oil/g protein, 29.00%, 93.10% - 69.05%, 6.25 m2/g and 29.27-87.81 min, respectively. Bands regarding CSPC in the 45 kDa molecular weight were detected by sodium dodecyl-sulfate polyacrylamide gel electrophoresis insert ignore into journalissuearticles values(SDS-PAGE); patterns. Fourier-transform infrared spectroscopy insert ignore into journalissuearticles values(FTIR); was used to verify the protein-specific structures. Sheet structures in the surface morphology of CSPC were dominant when scanning electron microscopy insert ignore into journalissuearticles values(SEM); images were investigated. Thermal gravimetric analyzer insert ignore into journalissuearticles values(TGA); results showed that the protein concentrate exhibited excellent stability to temperature.
Keywords : Pamuk tohumu protein izolatı, Tekno fonksiyonel özellikler, SDS page, Fourier dönüşümlü kızılötesi spektroskopisi, Taramalı elektron mikroskobu, Termogravimetrik analiz, Cottonseed protein isolate, Techno functional properties, Fourier transform infrared spectrop

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