IMPROVED EXTRACTION OF BIOACTIVE COMPOUNDS FROM THE POLLENS OF TYPHA DOMINGENSIS WITH SEQUENTIAL CONVENTIONAL AND ULTRASOUND TREATMENT

Typha domingensis pollen is obtained from staminate flowers of plant that is edible. In this study, bioactive compounds from pollen were extracted using ethanol and methanol with conventional method. Further ultrasonication was applied to the samples to improve extraction efficiency. The extraction yield was higher with ethanol insert ignore into journalissuearticles values(26.3±0.14%); than with methanol insert ignore into journalissuearticles values(25.4±0.3%); and applying ultrasonication increased the yields significantly insert ignore into journalissuearticles values(P <0.05);. Total phenolic compounds in ethanol and methanol extracts were determined as 9.83±0.48 and 9.71±0.55 mg GAE/g dry matter with the conventional method, and 11.76±0.64 and 12.74±0.37 mg GAE/g dry matter after ultrasonication, respectively. The flavonoid content with ethanol extraction was significantly higher than with methanol in both conventional and ultrasonication methods insert ignore into journalissuearticles values(P <0.05);. Antioxidant activities using DPPH, ABTS, CUPRAC, and FRAP tests, were determined, and enhanced antioxidant capacities were observed after ultrasonication. The bioactive compounds were qualitatively analyzed using UV-VIS spectroscopy and FTIR which confirmed the presence of polyphenols.