AN INVESTIGATION OF THE ACE INHIBITORY ACTIVITY, ANTIOXIDANT CAPACITY, AND PHYTOCHEMICAL CONSTITUENTS OF POLAR AND NON-POLAR EXTRACTS OF ZIZIPHUS JUJUBA FRUIT: STATISTICAL SCREENING THE MAIN COMPONENTS RESPONSIBLE FOR BIOACTIVITY

Authors : Bahar Fındık, Hilal Yıldız, Esma Birişçi, Serkan Yiğitkan, Pelin Köseoğlu Yılmaz, Abdulselam Ertaş

Pages : 554-566

Doi:10.15237/gida.GD24028

View : 79 | Download : 90

Publication Date : 2024-06-15

Article Type : Research Paper

Abstract :Herein, the angiotensin I-converting enzyme (ACE) inhibitory activity, antioxidant capacity, total polyphenol contents (TPC), and phytochemical profiles of polar and non-polar extracts of dried Ziziphus jujuba fruits were investigated, along with the statistical determination of the main components responsible for ACE inhibitory activity. The non-polar extract expressed the strongest ACE inhibitory activity (99.81%) among the extracts. The non-polar extract also exhibited the highest DPPH scavenging activity (IC50 of 30.63), linoleic acid/β-carotene bleaching capacity (89.31%), and TPC (59.47 mg GAE/g). The phenolic profiles of the extracts were identified by LC-MS/MS, and the presence of seven triterpenoid species in the extracts was examined using GC-MS techniques. The principal constituents included 19 phenolics, 2 organic acids, and 4 triterpenoids. A Pearson correlation and principal component analysis were conducted to find the correlation between individual phenolic compounds and ACE inhibitory activity.
Keywords : Ziziphus jujuba, ACE inhibitory activity, antioxidant capacity, phytochemical profile, Pearson correlation, principal component analysis

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