IMPACT OF ENVIRONMENTAL VARIABLES ON ANTIMICROBIAL SUBSTANCE PRODUCTION BY LACTIC ACID BACTERIA: A BOX-BEHNKEN DESIGN APPROACH

Authors : Evrim Güneş Altuntaş

Pages : 466-478

Doi:10.15237/gida.GD25018

View : 34 | Download : 38

Publication Date : 2025-06-10

Article Type : Research Paper

Abstract :In this research, we employed a 3-factor Box-Behnken experimental design (BBD) to ascertain the optimal conditions for the production of antimicrobial substances by lactic acid bacteria (LAB). The experimental parameters encompassed three variables: temperature (30, 35 and 37oC), incubation time (24, 48 and 72 hours), and substrate concentration (1%, 2% and 3%). The production of antimicrobial substances by Lactiplantibacillus plantarum F2 isolate was influenced by the incubation period. The highest antimicrobial substance production (average inhibition zone diameter 12.00 mm for antimicrobial activity assay; 12.09 mm for Box-Behnken estimation) of Pediococcus pentosaceus 50 isolate was achieved at a temperature of 37°C, an incubation period of 24 hours, and a substrate concentration of 2% in the environment. These findings indicate that the antimicrobial activity of LAB typically intensifies toward the end of the logarithmic phase and into the stationary phase, likely due to the increased production of secondary metabolites.
Keywords : Lactic acid bacteria, antimicrobial activity, optimization

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