Microbial sentinels in plant tissues: isolation and profiling of fungal and bacterial endophytes from diverse ecosystems of Sakarya Province

Authors : Muhammad Arif
Pages : 618-637
Doi:10.29050/harranziraat.1736913
View : 89 | Download : 160
Publication Date : 2025-12-17
Article Type : Research Paper
Abstract :Endophytes frequently occur in different plant tissues, both above-ground and belowground, of liverworts, hornworts, mosses, lycophytes, equisetopsids, ferns, and spermatophytes in natural forests and agricultural ecosystems. Specifically, the endophytes that are linked to the foliage of woody angiosperms are transmitted horizontally through the dispersal of sexual or asexual spores. Consequently, the identification of suitable endophytes for the purpose of biological control necessitates comprehensive investigation and comprehension of their interactions with the particular plant species and pathogens in question. In this study, a broad survey in 2023-24 and 2024-25 was conducted to find out the fungal and bacterial endophytes from the flora of Sakarya province. Healthy plant parts, including leaves, roots, tubers, stems, and fruits, were collected and brought to the laboratory for further analysis. Multiple growth media types were prepared to check the growth of both fungal and bacterial endophytes. The collected plant parts were washed and disinfected before placing them into the petri plates containing suitable media. All the specimens were incubated at 28±2°C. The emerging colonies of each endophyte were transferred to another petri plate to isolate the single spore for identification. Multiple prominent fungal and bacterial endophytic species were obtained from the flora of this province. There were multiple new species which were identified for the first time including fungi and bacteria. Maximum fungal and bacterial endophytic diversity was obtained from Arifiye and Serdivan from the horticultural species. The collected endophytic species will be tested as biological control agents against other plant pathogens associated with these areas.
Keywords : Endofitler, Sakarya, Bitki-mikrop etkileşimleri, Konak-spesifik endofitler, Mikrobiyal çeşitlilik, Ekoloji

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