Response Surface Methodology-Based optimization of Inulinase Production from New Bacillus Isolates

Authors : Sercan ÖZBEK YAZICI, Selmihan ŞAHİN, Bahadır TÖRÜN, Hacı Halil BIYIK, İsmail ÖZMEN

Pages : 1086-1101

Doi:10.16984/saufenbilder.897660

View : 42 | Download : 9

Publication Date : 2021-08-30

Article Type : Research Paper

Abstract :This study aimed to investigate new bacterial sources with the ability to produce inulinase and to optimize fermentation conditions for inulinase production. The inulinase production was carried out using newly isolated Bacillus licheniformis and Bacillus velezensis among identified sixteen bacterial strains. The four essential variables incubation time insert ignore into journalissuearticles values(1-3 days);, temperature insert ignore into journalissuearticles values(25-40°C);, pH insert ignore into journalissuearticles values(5-7);, and Wheat Bran insert ignore into journalissuearticles values(WB); level insert ignore into journalissuearticles values(1-5%); determined by the Plackett–Burman design insert ignore into journalissuearticles values(PB); were selected for optimization studies with Box-Benhken Design insert ignore into journalissuearticles values(BBD);. These parameters were found to be very effective on inulinase production. The maximum inulinase activities for B. licheniformis and B. velezensis were 401.18 EU/mL and 344.61 EU/mL. Both inulinases sustained 50% of their initial activity at 30°C for 9 days.. The results point out that bacteria are an important source as inulinase producer. The new isolates can be used in production of inulinase for industrial processes.
Keywords : Bacteria inulinase, response surface methodology, optimization, submerged fermentation

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