Production of Cellulase Enzyme by Pseudomonas reinekei SL7 Isolated from Paper Mill Wastes

Authors : Mehmet Akif Ömeroğlu

Pages : 15-21

Doi:10.46810/tdfd.1750168

View : 108 | Download : 168

Publication Date : 2025-12-30

Article Type : Research Paper

Abstract :The constantly growing energy requirements of today’s society and swiftly depleting fossil fuels indicate a need for a renewable alternatives. Cellulose is regarded as an alternative energy source and has recently become significant around the globe. Its obstinate structure must be degraded to utilize this valuable resource. Cellulose-degrading microorganisms are of great importance in the efficient use of cellulose resources. Screening of natural cellulolytic microorganisms and optimization of fermentation conditions are the focal points of research studies. Waste from paper mills is considered to be one of the main sources of cellulolytic bacteria. In the current study, isolation of cellulolytic bacteria was performed from paper mill wastes. After the determining the isolate showing the best cellulase activity, it was identified by sequencing the 16S rRNA gene region. According to the sequence analysis, it was detected that the isolate SL7 showed similarity to Pseudomonas reinekei with the rate of 99%. By optimizing the process parameters, the highest production of cellulase was obtained at temperature of 30 ⁰C, pH 7, carboxymethyl cellulose (CMC) concentration of 20 g/L, yeast extract concentration of 2 g/L and incubation time of 60 h. Under optimized conditions, the enzyme activity was detected to be 34.2 U/mL.
Keywords : Selülaz, Pseudomonas reinekei SL7, Kağıt Fabrikası Atıkları

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