Comparison of Glycated Hemoglobin Measurement with High Performance Liquid Chromatography and Immunoturbidimetry

Authors : Murat Can, Berrak Guven, Ferhan Adıbelli

Pages : 81-87

View : 46 | Download : 24

Publication Date : 2025-04-30

Article Type : Research Paper

Abstract :Aim: American Diabetes Association (ADA) have endorsed the use of HbA1c for diagnosing diabetes and for screening individuals at elevated risk for the disease. In our study, we aimed to evaluate the Lifotronic H9 High-Performance Liquid Chromatography (HPLC) Analyzer for the routine measurement of HbA1c in comparison to the Roche Cobas c501 method, which employs turbidimetric immuno-inhibition (TINIA). Material and Methods: A total of four hundred sixty-nine participants were included in the study. Among them, two hundred ninety-seven (63.3%) were identified as female, while one hundred seventy-two (36.7%) were male. Method comparison was done by linear regression and Bland-Altman plots. Results: HbA1c values obtained through the HPLC method were found to be greater than those obtained via the TINIA method, with measurements of 6.78±1.76 and 6.70±1.39, respectively. However, this difference was not statistically significant (p=0.461). The Passing–Bablok regression analysis indicated a slope of 0.90 (confidence interval (CI) 0.89-0.91) and an intercept of 0.59 (CI 0.50–0.67) for HbA1c, alongside a notably high correlation coefficient (r=0.974). Bland-Altman analysis conducted on repeated measurements across all patients indicated a strong level of agreement, characterized by a bias of +0.08% (CI 0.05–0.10). The analysis revealed that the two standard deviations (2 SD) established the lower limit at -0.47 (CI -0.52 to -0.43) and the upper limit at 0.63 (CI 0.59 to 0.68). Conclusion: The quality and performance of HPLC and TINIA are comparable and exhibit excellent correlation. Clinicians and clinical biochemists should collaborate on managing diabetes mellitus regarding diagnosis, treatment, and follow-up. This collaboration is essential for effective patient care.
Keywords : HbA1c, Türbidimetrik immün inhibisyon (TINIA), Yüksek Performanslı Sıvı Kromatografisi (HPLC), Lifotronic H9, Roche Cobas c501

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