The Effects of Althaea officinalis L. Extract on Cell Viability, Invasion, and Apoptosis in Prostate Cancer

Authors : Mustafa Said Atalay, Ayla Solmaz Avcıkurt, Sinem Gültekin Tosun

Pages : 457-465

Doi:10.32708/uutfd.1760397

View : 113 | Download : 287

Publication Date : 2025-12-08

Article Type : Research Paper

Abstract :This study aims to evaluate the cytotoxic effects of methanol (AOME) and ethyl acetate (AOEE) extracts obtained from the flowers of Althaea officinalis L., which is known for its high antioxidant capacity, on prostate cancer cell lines (LNCaP and PC3), as well as their inhibitory effects on cell migration and invasion, and their influence on pro-apoptotic gene expression. Cell viability was assessed using the MTT assay, while cell migration and invasion were analyzed using the scratch assay. Changes in pro-apoptotic gene expression were evaluated by real-time PCR (RT-qPCR), as determined at the IC₅₀ concentrations obtained from cytotoxicity tests. The results showed that AOME and AOEE induced dose-dependent cytotoxicity in both cell lines. The IC₅₀ values were found to be 1.1×10⁻³ μg/mL (AOME) and 11×10⁻⁵ μg/mL (AOEE) for PC3 cells, and 0.21×10⁻³ μg/mL (AOME) and 0.45×10⁻³ μg/mL (AOEE) for LNCaP cells. Scratch assay analyses demonstrated that both extracts significantly inhibited cell migration and invasion compared to the control group. Additionally, RT-qPCR analyses revealed that pro-apoptotic gene expression was significantly increased, and anti-apoptotic gene expression was significantly decreased in treated cells (p<0.05). These findings suggest that A. officinalis extracts possess properties that reduce cell viability, inhibit migration and invasion, and promote apoptosis in prostate cancer cells, indicating that the extract may have therapeutic potential against prostate cancer and warrant further validation through comprehensive molecular investigations.
Keywords : Althaea officinalis, apoptoz, prostat kanseri, yara iyileşmesi

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