Improving salt stress tolerance in Zea mays L. by modulating osmolytes accumulation and antioxidant capacity with Rutin

Authors : Asiye Sezgin Muslu

Pages : 21-29

Doi:10.30616/ajb.1387695

View : 113 | Download : 100

Publication Date : 2024-05-15

Article Type : Research Paper

Abstract :The growth and productivity of maize are severely affected by stress factors. Maize seedlings under salt stress were grown hydroponically to study the effect of rutin (Rut), a flavonoid, on changes in the stress parameters (thiobarbituric acid reactive substances (TBARS), hydrogen peroxide (H2O2), total chlorophyll), water status (leaf relative water content (RWC), osmolytes; proline, total soluble sugar), and activities of the main antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and peroxidase (POD)). After 21 days of growth, plants were applied with Rut as foliar spray. After 24 hours, seedlings were exposed to osmotic stress by 100 and 200 mM NaCI in the Hoagland’s Solution for 72 hours. Six groups were designed including a control (without NaCl or Rut), 150 mM NaCl, 200 mM NaCl, Rut, Rut+150 mM NaCl, and Rut+200 mM NaCl. Plant leaves were harvested 25 days after treatments. Exogenous significantly decreased TBARS and H2O2 contents in leaves of salt-stressed seedlings compared to salt stresses, enhanced the level of osmolytes, leaf RWC, activities of SOD, CAT, APX, and POD, and relative expression levels of SOD, CAT1, and APX1. As a result, findings from the study present reveal the effect of Rut on salt stress tolerance in maize seedlings under different osmotic stress. Here, it was clear that Rut played an active role in stress-alleviating. This application under salt stress can be useful in developing salt stress tolerance in crops for the agriculture sector.
Keywords : antioksidanlar, gen ifadesi, osmolitler, rutin, tuz stresi

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