The Effects of Angiotensin II, Olmesartan and PD123,319 on Protein Kinase C in STZ Induced Rat Vascular Smooth Muscle Cell Culture

Authors : Zehra ÇİÇEK, Kübra AKILLIOĞLU, Ayşe DOĞAN

Pages : 127-133

Doi:10.19127/bshealthscience.1141280

View : 36 | Download : 14

Publication Date : 2023-01-01

Article Type : Research Paper

Abstract :Protein kinase C insert ignore into journalissuearticles values(PKC); is a promoter enzyme that plays a vital role in signal transduction of vascular smooth muscle cells insert ignore into journalissuearticles values(VSMCs);. It has numerous vascular functions, such as vascular cell growth, cytokine activation and angiogenesis. But, these mechanisms are deteriorating in diabetes mellitus. Angiotensin II insert ignore into journalissuearticles values(Ang II); effects on vascular structure binding Ang II type-1 and type-2 receptors insert ignore into journalissuearticles values(ATR1 and ATR2); and stimulates PKC mostly through ATR1s. We aim to investigate the effects of Ang II, Olmesartan and PD123,319 on PKC levels of healthy and streptozotocin insert ignore into journalissuearticles values(STZ); induced rat VSMCs insert ignore into journalissuearticles values(H-VSMCs and STZ-VSMCs); in this study. The primary culture of VSMCs were isolated from thoracic aorta of healthy and STZ insert ignore into journalissuearticles values(45 mg/kg, given via the tail vein); induced Wistar rats. PKC levels were measured in H-VSMCs and STZ-VSMCs by enzyme-linked immunosorbent assay insert ignore into journalissuearticles values(ELISA);. In H-VSMCs, Ang II group compared to the control group, PKC levels decreased significantly insert ignore into journalissuearticles values(P = 0.000);; whereas, Ang II group compared to control, PKC levels were higher, but not significantly in STZ-VSMCs insert ignore into journalissuearticles values(P = 0.088);. PKC levels were increased in Ang II+Olmesartan insert ignore into journalissuearticles values(P = 0.000); and Ang II+PD123,319 insert ignore into journalissuearticles values(P = 0.000); groups compared to Ang II group in H-VSMCs, but in STZ-VSMCs, PKC levels in Ang II+Olmesartan insert ignore into journalissuearticles values(P = 0.001); and Ang II+PD123,319 insert ignore into journalissuearticles values(P = 0.000); groups compared to Ang II group were decreased significantly. Ang II, its receptors and PKC seem to modulate each other and may have a relationship in hyperglycemic conditions. Also, considering that, ATR1 blocker insert ignore into journalissuearticles values(ATR1B); Olmesartan and ATR2 blocker insert ignore into journalissuearticles values(ATR2B); PD123,319 may be protective against vascular injury by reducing PKC levels in STZ-VSMCs.
Keywords : Ang II, PKC, Vascular smooth muscle, Cell culture

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