Comparison Of the Effectiveness of Density Gradient Centrifugation and Microchip Sperm Preparation Methods On Pregnancy Success In Intrauterine Insemination

Authors : Bihter Senem Feyzioğlu, Zerrin Avul

Pages : 37-42

View : 61 | Download : 31

Publication Date : 2024-07-29

Article Type : Research Paper

Abstract :Introduction: Intrauterine insemination technique is one of the most commonly used methods in assisted reproductive treatments. Semen parameters are one of the prognostic factors affecting the clinical success of the intrauterine insemination method, and different sperm preparation techniques are applied to select quality sperm. Methods: In this retrospective study, 217 couples who applied to the infertility center of our hospital between 2012 and 2020 were included in the study. Before the application of the intrauterine insemination method, semen samples taken from 178 patients were prepared by microchip method and semen samples taken from 39 patients were prepared by density gradient centrifugation method, and the concentration and motility properties of sperm were evaluated. Results: The clinical pregnancy success of sperm samples prepared with microchip and density gradient centrifugation method was evaluated after intrauterine insemination application. In the density gradient centrifugation method, motile sperm (79.46±12.48%) were obtained at a higher rate than the microchip method (57.48±20.24). While clinical pregnancy was observed in 46.15% of the patients included in the density gradient centrifugation group, it was determined as 23.03% in the microchip group. On the other hand, while pregnancy continued in 27.78% of the density gradient centrifugation group patients, the continuing pregnancy in the microchip group was determined as 53.66%. As a result, while the percentage of motile sperm and the incidence of clinical pregnancy increase in the density gradient centrifugation method compared to the microchip method, there is a significant decrease in the ongoing pregnancy rates. Conclusions: It is seen that sperm samples prepared by microchip method have positive contributions to the clinical success of intrauterine insemination.
Keywords : İnfertilite, Intrauterin inseminasyon, Dansite gradiyent santrifügasyon, Mikroçip, Sperm hazırlama

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