Identification of strain types of some Beet necrotic yellow vein virus isolates determined in Northern and Central Parts of Turkey

Authors : Nazli Dide Kutluk Yilmaz

Pages : 241-248

Doi:10.18393/ejss.2016.3.241-248

View : 51 | Download : 14

Publication Date : 2016-06-20

Article Type : Research Paper

Abstract :Beet necrotic yellow vein virus insert ignore into journalissuearticles values(BNYVV);, the agent of rhizomania disease, causes severe economic losses in sugar beet fields in all over the world. The virus is transmitted by a plasmodiophorid vector, Polymyxa betae Keskin. Twenty soil samples, collected from sugar beet fields in northern and central parts of Turkey during surveys in 2004 and 2005 and known to be infested with viruliferous cultures of P. betae carrying BNYVV, were selected and used in this study. Sample selection was made according to symptom expression of beet seedlings in preliminary bait plant tests and locations of the soil samples that accurately represent the region from which they were taken. Total RNAs were extracted from sugar beet plants grown in these soils and used to amplify RNA-2 insert ignore into journalissuearticles values(nt. 19-1088); and RNA-3 insert ignore into journalissuearticles values(nt. 50-1268); of BNYVV by reverse transcription polymerase chain reaction insert ignore into journalissuearticles values(RT-PCR); method. Restriction fragment length polymorphism insert ignore into journalissuearticles values(RFLP); analysis of PCR-amplified products showed that most of BNYVV isolates studied were A-type strain, however, two isolates did not exactly match the band profile of A-type strain. Additionally, the presence of BNYVV RNA-5 component was investigated by RT-PCR using the primers specific for P26 coding region. Four samples belonging to three provinces were found to be involving RNA-5 segment insert ignore into journalissuearticles values(20%);.
Keywords : Sugar beet, rhizomania, RFLP, RT PCR

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