Effect of bio-fertilizers application on microbial diversity and physiological profiling of microorganisms in arable soil

Authors : Soňa JAVOREKOVÁ, Jana MAKOVÁ, Juraj MEDO, Silvia KOVÁCSOVÁ, İvana CHAROUSOVÁ, Ján HORÁK

Pages : 54-61

Doi:10.18393/ejss.07093

View : 78 | Download : 12

Publication Date : 2015-01-08

Article Type : Research Paper

Abstract :In laboratory assay, the diversity of bacteria and microscopic fungi and the community-level physiological profiling insert ignore into journalissuearticles values(CLPP); of microorganisms were observed after the addition of bio-sludge insert ignore into journalissuearticles values(40 t.ha -1 ); from a biogas station and addition of bio-fertlizers - AZOTER insert ignore into journalissuearticles values(10 dm -3 .ha -1 ); to the arable soil with PCR-DGGE and BIOLOG ® method insert ignore into journalissuearticles values(Eco Plates);. The differences were recorded in the microbial diversity insert ignore into journalissuearticles values(bacteria and microscopic fungi); among variants according to the Shannon index. The differences in community of microscopic fungi were markedly higher among the soil samples with the additions of both  bio-fertilizers  compared to  control soil samples. The occurrence of individual OTUs insert ignore into journalissuearticles values(operational taxonomic units); bacteria and microscopic fungi were different after 105 days of incubation from the status after the 1 st day of incubation. The community metabolic diversity insert ignore into journalissuearticles values(CMD); was influenced by the incubation time insert ignore into journalissuearticles values(105 days); as well, but not by application of bio-fertilizers. We observed a significant decrease insert ignore into journalissuearticles values(LSD test, P <0.05); in community metabolic diversity insert ignore into journalissuearticles values(CMD); and average metabolic response  insert ignore into journalissuearticles values(AMR); of microorganisms   in samples collected on the 105 th day of the experiment compared to  samples collected on the 1 st day of the experiment in all tested samples.
Keywords : bio fertilizers, microbial diversity, microbial physiological profiling, arable soil

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