Green Synthesis of Silver Nanoparticles from Phaseolus vulgaris L. Extracts and Investigation of their Antifungal Activities

Authors : Ege EDİZ, Gülbin KURTAY, Başar KARACA, İlker BÜYÜK, Fatma Şeyma GÖKDEMİR, Sumer ARAS
Pages : 11-23
Doi:10.15671/hjbc.680371
View : 39 | Download : 10
Publication Date : 2021-01-01
Article Type : Research Paper
Abstract :In this study, we aimed to synthesize silver nanoparticles insert ignore into journalissuearticles values(AgNPs); using leaf, root, and stem extracts of Phaseolus vulgaris L. insert ignore into journalissuearticles values(Yunus-90); and elucidate their antifungal activities. In this regard, the prepared AgNPs have been characterized by using UV-vis, FT-IR, TEM, SEM, and DLS techniques. Then, the antifungal activity of both synthesized and commercially purchased AgNPs was investigated via insert ignore into journalissuearticles values(i); agar well diffusion, insert ignore into journalissuearticles values(ii); fungal colony morphotype diversity, insert ignore into journalissuearticles values(iii); inhibition of hyphae and insert ignore into journalissuearticles values(iv); minimum inhibition concentration insert ignore into journalissuearticles values(MIC); analyses against Colletotrichum sp. , Fusarium oxysporum , Fusarium acuminatum , Fusarium tricinctum , Fusarium graminearum , Fusarium incarnatum , Rhizoctonia solani , Sclerotinia sclerotiorum , and Alternaria alternata . The AgNPs derived from the leaf extract displayed significantly higher levels of antifungal activity relative to the AgNPs prepared from the root and stem extracts. The commercial AgNPs also displayed lower antifungal activity than their green equivalents synthesized in this research, and even a low insert ignore into journalissuearticles values(~50 μg/mL); concentration of synthesized AgNPs was found to be effective in suppressing the growth of Fusarium tricinctum and Colletotrichum sp.
Keywords : Phaseolus vulgaris L, Green synthesis, Silver nanoparticle AgNP, Antifungal activity

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