In Vitro Effects of Dexamethasone on Human Serum Paraoxonase-I PON1 Activity

Home Page
About
Submit A Journal
Submit A Conference
Submit Paper/Book
- Submit a Preprint
- Submit a Book
Contact

Hacettepe Journal of Biology and Chemistry
Volume:37 Issue:3
In Vitro Effects of Dexamethasone on Human Serum Paraoxonase-I PON1 Activity

In Vitro Effects of Dexamethasone on Human Serum Paraoxonase-I PON1 Activity

Authors : Şükrü BEYDEMİR, Deniz EKİNCİ, Orhan ATEŞ

Pages : 197-205

View : 38 | Download : 8

Publication Date : 2009-08-01

Article Type : Research Paper

Abstract :Dexamethasone has successfully been used for treatment of some inflammatory diseases. Here, we evaluated the in vitro effects of dexamethasone on the activity of human serum paraoxonase hPON1 . PON1 enzyme was purified using simple chromatographic methods: DEAE-Sephadex anion exchanger and Sephadex G 200 gel filtration chromatography from human serum. hPON1 was purified approx. 225-fold with a final specific activity of 4867.3 U x mg-1 proteins and with a purity up to 39.8%. Dexamethasone dose-dependently decreased in vitro hPON1 activity. IC50 value for dexamethasone was determined as 1.106. Inhibition constant Ki was estimated as 3.284 ± 0.394 mM from Lineweaver-Burk graph. Inhibition type of dexamethasone was determined as noncompetitive. Our results show that dexamethasone inhibits hPON1 activity under in vitro conditions.
Keywords : Paraoxonase, Dexamethasone, Human serum, Inhibition, Enzyme purification

ORIGINAL ARTICLE URL

* There may have been changes in the journal, article,conference, book, preprint etc. informations. Therefore, it would be appropriate to follow the information on the official page of the source. The information here is shared for informational purposes. IAD is not responsible for incorrect or missing information.