Development of a UV-Vis Spectroscopic Method for Etodolac Determination and Evaluation of Pharmaceutical Additive Effects

Authors : Hosna Adib, Iraj Karimpour, Sevda Akay Sazaklioglu

Pages : 178-186

Doi:10.38001/ijlsb.1717622

View : 54 | Download : 146

Publication Date : 2025-12-23

Article Type : Research Paper

Abstract :Etodolac is an active substance from the nonsteroidal anti-inflammatory drug (NSAID) class and is widely used in the treatment of pain and inflammation. In this study, a simple, rapid, and sensitive UV-Vis spectrophotometric method was developed and validated for the quantitative determination of etodolac from commercial tablet formulations. Etodolac showed maximum absorbance at 278 nm. Methanol was optimized as the most suitable solvent in the study, and the method was validated according to these conditions. The method demonstrated a linear working range of 0.88- 40 µg mL-1. The limit of detection (LOD) was found to be 0.29 µg mL-1. Additionally, the effects of some pharmaceutical excipients on the UV absorbance of etodolac were investigated. Pharmaceutical excipients can cause changes in the UV absorbance of the active ingredient, which can lead to incorrect dosage determinations and analytical errors. In this context, the effect of mannose, lactose monohydrate, and potassium sorbate on the maximum absorbance of etodolac at 278 nm was evaluated, and possible interferences were examined. In addition, stability studies were carried out for four weeks to test the long-term reliability of the analysis method. Moreover, the recovery of etodolac from the Etotio tablet formulation was measured, and the method\\\'s accuracy was evaluated. The data showed that the applied analysis method offers high precision, reproducibility, and reliability. This study presents an optimized, sensitive, and selective method for accurately quantifying etodolac in pharmaceutical preparations.
Keywords : Etodolak, Analitik yöntem, UV-Vis spektroskopisi, İlaç analizi, İlaç katkı maddeleri

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