Bismuth oxide nanoparticles induced oxidative stress-related inflammation in SH-SY5Y cell line

Authors : Ezgi OZTAS, Mahmoud ABUDAYYAK, Beyza AYKANAT, Zubeyde CAN, Enes BARAM, Gul OZHAN

Pages : 173-179

View : 45 | Download : 14

Publication Date : 2019-12-01

Article Type : Research Paper

Abstract :DOI : 10.26650/IstanbulJPharm.2019.19020 Bismuth insert ignore into journalissuearticles values(III); oxide nanoparticles’ insert ignore into journalissuearticles values(Bi2O3-NPs); unique physicochemical properties attracted the attention in biological, industrial, technological and medical fields. Concurrently, increasing numbers of studies revealing their potential toxic effects and possible toxicity mechanisms are ongoing. In this study, we assessed the toxic potentials of Bi2O3-NPs in human SH-SY5Y neuroblastoma cell line. After Bi2O3-NPs characterization using TEM, the cytotoxic potentials were evaluated by MTT and LDH assays. The induction of reactive oxygen species production was evaluated by H2DCFDA. In order to evaluate the oxidative damages, the changes in antioxidant catalase and superoxide dismutase and glutathione levels were determined. The cellular death pathway and the role of immune response were studied by measuring the mRNA expression levels of related genes. Our results showed that Bi2O3-NPs decreased the cell viability through disruption on mitochondrial activity insert ignore into journalissuearticles values(IC50:77.57 μg/mL); and membrane integrity insert ignore into journalissuearticles values(LDH%50:16.97 μg/mL);. At 50 μg/mL Bi2O3-NPs, the production of reactive oxygen species insert ignore into journalissuearticles values(ROS); was induced significantly as well as the catalase and superoxide dismutase levels. In immune response, the mRNA expression levels of interleukin insert ignore into journalissuearticles values(IL);-6 were increased more than 1.5-fold in all doses; whereas, TNF-α, NF-ĸB and MAPK8 expressions were remained unchanged. Consequently, Bi2O3-NPs induced oxidative stress-related inflammation via activation of pro-inflammatory cytokine, IL-6. You may cite this article as : Öztaş E, Abudayyak M, Aykanat B, Can Z, Baram E, Özhan G insert ignore into journalissuearticles values(2019);. Bismuth Oxide Nanoparticles Induced Oxidative Stress-Related Inflammation in SH-SY5Y Cell Line. Istanbul J Pharm 10.26650/IstanbulJPharm.2019.19020.
Keywords : Bismuth III, oxide nanoparticles, Neurotoxicity, Oxidative stress

ORIGINAL ARTICLE URL