The cytotoxicity and insecticidal activity of extracts from Delphinium formosum Boiss. & Huet

Authors : Burcu SENUTSUKARCİ, Nurhayat TABANCA, Alden S ESTEP, Ozlem AKBALDAGİSTAN, Sonja M KESSLER, Zehra OZTURK, James BECNEL, Alexandra K KİEMER, Afife MAT

Pages : 148-153

View : 34 | Download : 10

Publication Date : 2019-12-01

Article Type : Research Paper

Abstract :DOI : 10.26650/IstanbulJPharm.2019.19034 Delphinium species are well-known toxic plants with their diterpenoid alkaloid contents. There has been no previous investigation on the cytotoxicity of Delphinium formosum Boiss. & Huet. The extracts of the different parts of D. formosum, an endemic species in Turkey, were investigated for their cytotoxic activity against the human liver carcinoma cell line insert ignore into journalissuearticles values(HepG2); and primary human umbilical vein endothelial cells insert ignore into journalissuearticles values(HUVEC);. The cytotoxic effects of twelve extracts and subfractions were determined against HepG2 cells using MTT assay. The only active extract was applied to the HUVEC as a model for healthy cells. Only one of the alkaloid-containing extracts from the aerial parts was toxic insert ignore into journalissuearticles values(IC50 = 244,9 μg/mL against HepG2 and 144,4 μg/mL against HUVEC);, while the root extracts were inactive. The results were improbable although it’s predicted secondary metabolites, such as diterpene alkaloids insert ignore into journalissuearticles values(methyllycaconitine, browniine, lycoctonine, avardharidine, antranoyllycoctonine, delsemine A/B and lycoctonine);. Based on previous studies in literature, the cytotoxic plants were also expected to exhibit insecticidal activity. Therefore, the cytotoxic extract of D. formosum was examined for its adulticidal and larvicidal activity against the yellow fever, dengue fevers and Zika virus vector Aedes aegypti L. You may cite this article as : Sen-Utsukarci B, Tabanca N, Estep AS, Akbal-Dağıstan Ö, Kessler SM, Öztürk Z, Becnel J, Kiemer AK, Mat A insert ignore into journalissuearticles values(2019);. The Cytotoxicity and Insecticidal Activity of Extracts from Delphinium formosum Boiss. & Huet. Istanbul J Pharm 10.26650/IstanbulJPharm.2019.19034.
Keywords : Delphinium formosum, cytotoxic activity, HepG2, HUVEC, natural insecticidal, Aedes aegypti

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