Determination of sultamicillin in bulk and pharmaceutical preparations via derivatization with NBD-Cl by spectrophotometry

Authors : Elif ÖZDEMİR, Duygu TAŞKIN, Sıdıka SUNGUR

Pages : 1-5

View : 38 | Download : 13

Publication Date : 2020-04-01

Article Type : Research Paper

Abstract :Background and Aims: A new sensitive and selective method for the determination of sultamicillin in bulk and pharmaceutical preparations has been developed. Methods: The developed method is based on the derivatization of sultamicillin with 4-chloro-7-nitrobenzofurazan in pH 9.0 borate buffer at 70ºC for 60 min. The derivative was measured spectrophotometrically at 432 nm after extraction with chloroform. Results: The method was validated by linearity, LOD, LOQ, accuracy, precision and recovery parameters. The linear range for sultamicillin was 10-50 µg.mL-1 with a correlation coefficient of 0.9999. The limit of detection insert ignore into journalissuearticles values(LOD); and limit of quantification insert ignore into journalissuearticles values(LOQ); were found to be 1.47 and 4.41 µg/mL-1, respectively. Conclusion: The suggested method was validated, and can be applied for routine sultamicillin analysis in dosage formsfor industrial analysis.
Keywords : 4 chloro 7 nitrobenzofurazan, sultamicillin, spectrophotometry, validation

ORIGINAL ARTICLE URL