Determination of chlorpheniramine enantiomers in pharmaceutical formulations by HPLC on chiral column with PDA detection

Authors : Gizem ERENSOY, Duygu TAŞKIN, Gamze ÖZGÜL ARTUÇ, Elif ÖZDEMİR, Sumru ÖZKIRIMLI

Pages : 149-152

View : 48 | Download : 4

Publication Date : 2020-08-27

Article Type : Other Papers

Abstract :Background and Aims: An HPLC method with photodiode array detector on a chiral column was proposed for enantioselective determination of chlorpheniramine insert ignore into journalissuearticles values(CLP); enantiomers in dosage forms. Methods: The enantioselective determination was achieved on amylose trisinsert ignore into journalissuearticles values(3,5-dimethylphenylcarbamate); column, using n-hexane-insert ignore into journalissuearticles values(propan-2-ol);-diethylamine insert ignore into journalissuearticles values(97.5:2.5:0.025, v/v/v); mobile phase. The peaks were detected at 258 nm. Diphenhydramine was used as an internal standard insert ignore into journalissuearticles values(IS);. A new sample preparation procedure was developed to avoid the interference of the other ingredients present in the formulations. Results: Limit of quantification of the proposed method was 0.88 and 1.31 μg/mL for S-insert ignore into journalissuearticles values(+);-CLP and R-insert ignore into journalissuearticles values(-);-CLP, respectively. Conclusion: The method is linear, sensitive, specific and can be used for the enantioselective assay of CLP enantiomers in pharmaceutical formulations.
Keywords : Chlorpheniramine, amylose tris 3, 5 dimethyl phenylcarbamate, enantioselective determination, HPLC PDA, chirality

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