- İstanbul Journal of Pharmacy
- Volume:51 Issue:1
- In vitro urease and trypsin inhibitory activities of some sulfur compounds
In vitro urease and trypsin inhibitory activities of some sulfur compounds
Authors : Eda DAĞSUYU, Refiye YANARDAĞ
Pages : 85-91
View : 39 | Download : 11
Publication Date : 2021-04-30
Article Type : Research Paper
Abstract :Background and Aims: Organosulfur compounds modulate the activities of plurality of metabolic enzymes, especially those that activate insert ignore into journalissuearticles values(cytochrome P450s); or detoxify insert ignore into journalissuearticles values(glutathione-S-transferases); carcinogens. They also inhibit the formation of DNA adducts in different target tissues. The aim of the present study was to investigate the effect of some sulfur compounds on urease and trypsin activities in vitro. Methods: In the present study, the inhibitory effect of sulfur compounds on the activities urease and trypsin were determined according to the method of Hanif et al. insert ignore into journalissuearticles values(2012); and Ribeiro et al. insert ignore into journalissuearticles values(2010);, respectively. Results: In comparison to the reference standard thiourea insert ignore into journalissuearticles values(IC50= 53.81±0.68 μg/mL);, S-allyl-L-cysteine insert ignore into journalissuearticles values(IC50= 0.88±0.01 μg/ mL); and D, L-methionine insert ignore into journalissuearticles values(IC50= 0.91±0.02 μg/mL); had the highest urease inhibitor activity, corresponding to the lowest IC50 values among the sulfur compounds. Among the sulfur compounds used in this study, D,L-methionine insert ignore into journalissuearticles values(IC50= 0.13±0.01 mg/ mL); exhibited the lowest IC50 value for trypsin inhibitor, though its activity was less than that of tannic acid which was used as a standard insert ignore into journalissuearticles values(IC50= 0.06±0.01 mg/mL);. Conclusion: The present outcome suggests that sulfur compounds are potential inhibitors of urease and trypsin activities, and may find importance in medicine and agriculture.Keywords : Enzyme, inhibitors, sulfur compounds, urease, trypsin
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